Coding

Part:BBa_M36762:Design

Designed by: Andrew Lee, Clinton Olivas, Daniel Hu   Group: Stanford BIOE44 - S11   (2014-10-23)


Chicken Lysozyme C (LYZ) conjugated with Albumin Signal Peptide


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 215
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 434
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We checked to make sure the sequence did not include any BsaI restriction sites. Given that the signal peptide is derived from mammalian cells, it is uncertain whether or not the yeast will read the peptide and secrete the lysozyme properly from the cytosol, despite it being yeast optimized.


Source

The gene comes from chicken lysozyme C, coded by the LYZ gene. The signal peptide comes from Oxford Genetics optimized yeast lysozyme secretion vector, pSF-TEF1-NH2-Lys.

References